Background: Health care associated infections are an important risk factor that increases mortality and morbidity. Care bundles are used ever more frequently in order to eliminate patient care associated infections at intensive care units (ICU). \nMethods: In this study, the data of the patients were analyzed retrospectively who were treated at the ICU of a university hospital that uses the ventilator associated pneumonia care bundle, urinary catheter associated urinary system infection care bundle and central venous catheter associated blood circulation infection care bundle. In the study, 5967 sickness days of 1617 patients were evaluated; catheter usage periods and infection rates were investigated.\nResults: For the time the patients spent in the ICU, urinary catheters were used 87.6% of the time, ventilator catheters were used 35.9% of the time and central venous catheters were used 49.7% of the time. While in 2013 urinary system infection was detected in one patient (infection rate 0.18), ventilator-related pneumonia was detected in 1 patient (infection rate 0.18), blood circulation infections were detected in 7 patients (infection rate 1.30), and no infections were detected at the ICU in 2014 and 2015.\nDiscussion: Care bundles that were prepared based on evidence at the intensive care units are a method, designed to improve patient care processes which decreases catheter associated infection rates and hospital costs. Preparation and application of care bundles by establishing infection control teams at hospitals, plays an important role in fighting infections. In our study, early period results of ventilator, urinary catheter and central venous catheter care bundles used in the intensive care unit of a university hospital were examined, and the goal of zero infection was achieved at the second year of care bundle application. \nConclusions: It is not impossible to achieve the aim of zero infection upon the application of care bundles at intensive care units and by inspection of application by the authorized staff.