Parents of children with autism may suffer high levels of stress, resulting in disruption in lifestyle and relationship, deprivation of human need, and failure to act in ways to eliminate the cause of the distress. Moreover, family stress can contribute to unfavorable prognosis. This study aimed to assess stressors and needs of parents having children with autism at Al-Taif city. Design: A descriptive study design was used. Setting: The study was conducted at the Special Needs Health Care Center and Al-Rawda18 nursery school in Al-Taif city. A convenient sample was used in the study. It included 101 parents and their autistic children. Three tools were used to collect the data: the Child\'s Medical and Developmental Data Sheet, the Parenting Stress Index, and resources/needs inventory. The study findings of the present study revealed that (48%) of children had initial diagnosis of autism, (48%) of their mothers had university education. (85.1%) of children were born full term and parents accepted pregnancy (82.2%) More than half were born normally (66.3%). A statistically significant relationship was found between parents\' stressors and their needs. It can be concluded that almost all parents of autistic children have high levels of stressors. Most children with autism have a family history of neurological and mental problem. The study recommended that Nurses should exert more effort to support parents of children with autism through counseling clinics. Future researches are proposed to investigate the effectiveness of nursing interventions aimed at enhancing the use of coping strategies among parents with autistic children.

Sulfamethoxazole and trimethoprim (SXT) block the synthesis of tetrahydrofolate, inhibiting bacterial growth. An increase in the number of plasmids that carry genes which code for resistance to both of these antimicrobial agents has been reported. In Venezuela many of the Escherichia coli strains cause urinary infections, and are multiresistant to the antibiotics commonly used to treat them. In this study we isolated E. coli strains that cause urinary tract infections and are resistant to SXT from outpatients. Antibiotic resistance profile was determined. The presence of plasmid molecules was determined using bacterial conjugation assays; these were then purified and treated with restriction enzymes. Overall, 358 samples from patients were analyzed, of which 64% were positive for bacterial growth. E. coli represented 67% of the bacterial population, of which 32% showed resistance to SXT, and of these 83% were resistant to AMP. Twenty eight percent of the SXT resistance found was transferred by conjugation. Plasmids treated with EcoRI showed different restriction patterns. This is the first study done in Venezuela that has identified the genetic entity responsible for harboring SXT resistant genes, as well as determining that there is no apparent relation between the different plasmid carriers.