Aim: This study aimed at analyzing the internal reliability of the Knowledge and Attitude Scale for Geriatric Functional Status Assessment which was developed for measuring the information and attitudes of primary care physicians in terms of geriatric functional status assessment.\n Method: The study participants were 41 physicians working in 19 Family Health Centers in Edirne city center between the dates of 15.09.2015-15.10.2015. Data was collected by using a scale which was prepared by our department by using the information in literature. The scale contained a total of 22 questions including 9 questions on socio-demographics and 13 questions in the form of 5-point Likert scale. Data was evaluated by using SPSS program, and Cronbach’s alpha reliability analysis was made.\n Results: Of the participating physicians, 28 were male (68.3%) and 13 were female (31.7%). Their average score was 24.32±11.50. We found a significant relation between physicians’ having training on functional status assessment and knowing how to make a geriatric functional status assessment (Pearson χ2=33.218; p<0.01). According to the reliability analysis of the 13-item scale, the Cronbach’s alpha value was 0.934. \n Conclusion: The scale’s reliability was proved for measuring the information and attitudes of primary care physicians in terms of geriatric functional status assessment in our country.
Chlamydomonas reinhardtii has been accepted as an important and potential algal species for heterologous protein production in the recent days. A vast amount of genetic information and various recently developed genetic techniques and strategies shows future potentiality of this algal species as industrial recombinant protein production platform. Among the three genomes of this microalgae, nucleus could be more potential than other genomic compartments for recombinant protein production due to secretion signal which helps to secret protein into exterior media. Although, the poor expression of transgene is a great challenge of this protein production platform. But, the recent advancement of molecular tools to interrogate and edit the nuclear genome including the development of new nuclear selection marker, reporter genes, secretion signals and tagging approaches of target protein increases the potentiality of the platform as well. Till now, different review papers have been published addressing specific issues related to either transgene expression or construction of advanced molecular vector to initiate the protein production. This review aims to address these issues together in a single paper to make a up to date picture of the development of nuclear compartment of C. reinhardtii as industrial recombinant protein production platform. In addition, the future direction of the research towards the development of this genomic compartment as an industrial recombinant protein production platform will be discussed here.