Our study aims to show whether the administration of Fibroblast Growth Factor-2 (FGF2) and ozone against neuron-damaging hypoxia-ischemia has any neuroprotective effects in rats exposed to experimental cerebral ischemia-reperfusion. 6 groups were created with each group containing 10 rat that 7d old male Wistar rats. The first group, which was the Sham group, was the one in which a neck dissection was performed while ischemia was not caused. 2nd group, carotid arteries were linked, hypoxia was induced in order to cause hypoxia-ischemia (HI), which is done all groups except sham. The other groups were the ones with 10 µl/ml and 20 µl/ml FGF2 administered. The last groups were the ones with 25 ?g/ml and 50 ?g/ml ozone administered, routine tissue follow-up procedures were carried out and stained with hematoxylin and eosin stain, and inflammation, eosinophilic cytoplasm, edema in the cerebral cortex, vascular congestion and necrobiotic-necrotic changes were scored and evaluated. In light microscope revealed moderate-to-severe eosinophilic cytoplasm and necrobiotic changes in the HI group when compared to the Sham group and the 20 µl/ml FGF2 group among others. Significant differences in the criteria of inflammation, vascular congestion and edema in the cerebral cortex was detected between the same groups. Kruskal-Wallis test was used for intergroup comparison resulted in a statistically significant difference between all of the study groups (p< 0.05). In our study, morphological recovery was observed depending on the different doses of FGF2 and ozone used to protect the neurons, were fund to be statistically significant.
The aim of this study was to investigate effect of the compressive follower preload on modal characteristics of the human whole lumbar spine. A computed tomography (CT)-based 3D ligamentous FE model of the human T12–pelvis spinal segment was developed and validated to predict lumbar modal responses in terms of resonant frequency and mode shape in vertical direction under different values of compressive follower preload. The results showed that under the 0 N, 400 N, 600 N and 800 N preloads, the vertical resonant frequencies for the T12–pelvis model were 6.49 Hz, 7.27 Hz, 7.55 Hz and 7.76 Hz respectively, which presented an increase with increasing preload magnitude. The mode shapes at these resonant frequencies illustrated that the lumbar spine not only performed vertical motion but anterior-posterior (A-P) motion and rotation under vertical vibration. Moreover, increasing the preload decreased vertical displacement of the vertebrae and also altered their A-P displacement and rotational angle in the sagittal plane for the vertical mode shape.