Abstract \nBackground: The National Tuberculosis guidelines indicate that management of maternal Tuberculosis should follow a similar path to the treatment for other people. Maternity guidelines in South Africa stipulate that midwives should perform TB screening prior to conception, during pregnancy as well as a 42-day puerperium period. \nObjectives: The purpose of the study was to assess the knowledge and practice of midwives in management of maternal Tuberculosis. \nMethods: A quantitative cross-sectional descriptive design was used to assess the knowledge and practices of midwives. Anon-probability purposive sampling approach was used to select 103 midwives from ten hospitals in the Limpopo Province, South Africa. Data were collected through self-administered questionnaire with closed questions. Data were analysed using Statistical Package of Social Sciences v24.00. Issues of ethical consideration, validity and reliability were maintained throughout the study. \nResults: The findings revealed that midwives lack knowledge and practice related to the National Tuberculosis Guidelines and TB screening is not carried out to majority of pregnant women during their first presentation at antenatal care services. \nConclusions: Recommendations are to conduct in-service training workshop and seminars for midwives in a National Tuberculosis programmes.