Diabetics have been recognized as a leading cause for male infertility. This study was carried out to investigate reproductive disorders caused by diabetics and the possible prevention through the use of lemongrass, marjoram and thyme extract at two levels 250 and 500 mg/kg body weight in rats for biological experimental. Chemical constituents, minerals content and fractionation of polyphenol compounds using High Performance Liquid Chromatography (HPLC) were determined in lemongrass, marjoram and thyme. The results showed that these herbs had riches amounted from protein, mineral content and polyphenol compounds as natural antioxidants.\nAt the end of experiential biological evaluation the sex organs (testis, seminal vesicle and vas deferens) weights, epididymis sperm count, hormonal profile, serum glucose, serum lipids, measurement of aromatase activity, assay of 3b-Hydroxysteroid dehydrogenase (3b-HSD) activity, acid phosphatase (ACP) and alkaline phosphatase (ALP) were estimated in diabetic rats had taken orally daily of lemongrass, marjoram and thyme extract at level 250 and 500 mg/kg b.w. compared with negative control (healthy rats) for four weeks.\nThe results observed that no significant changes in sex organs between diabetic rats had taken from lemongrass, marjoram and thyme extract orally daily at level 500 mg/kg b. w. and negative control rats. From the results it could be noticed that increased serum levels of leptin, prolactin (PRL) and estrogen (E2), with increased serum dehydroepiandrosterone (DHEA) and testosterone (T) were also observed. Additionally, the results showed significant increase in epididymal sperm count, as well as in steroidogenic enzymes; 3b-hydroxysteroid dehydrogenase (3b- HSD), alkaline phosphatase (ALP), and acid phosphates (ACP), with marked elevation in aromatase activity in testis of the diabetic rats. Oral administration of lemongrass, marjoram and thyme extracts at level 500 mg/kg b.w seemed to prevent overall mentioned alterations, as evident by elevated sperm count, in addition to improved testicular structure.\nFrom the obviously results it could be recommended that the lemongrass, marjoram and thyme extract had riches amounted polyphenol compounds and when give the diabetic rats lemongrass, marjoram and thyme extract at level 500mg/kg body weight may be beneficial for diabetic rats who have fertility problem, as their extracts produce antidiabetic activity and exhibit fertility enhancing properties in diabetic rats.